We evaluate and optimize 5' and 3' untranslated regions (UTRs) to enhance mRNA stability and translation efficiency. Our UTR libraries are screened in vitro for optimal expression profiles across various cell types.
We evaluate and optimize 5' and 3' untranslated regions (UTRs) to enhance mRNA stability and translation efficiency. Our UTR libraries are screened in vitro for optimal expression profiles across various cell types.
Using proprietary algorithms and context-specific analysis, we optimize coding sequences for enhanced expression, stability, and reduced immunogenicity in mammalian systems.
Our experts design and build custom plasmids with your gene of interest, optimized regulatory elements, and necessary transcriptional controls to ensure high-yield mRNA production.
We produce high-purity mRNA using enzymatic IVT, incorporating cap structures (Cap 0, Cap 1), modified nucleotides (e.g., pseudouridine), and poly(A) tails tailored to your specifications.
All mRNA batches undergo rigorous QC, including:
HPLC for purity and modified nucleotide incorporation
Fragment Analyzer for size distribution and integrity
UV spectrophotometry and gel electrophoresis for yield and confirmation
We test the functionality of your mRNA in relevant cell lines to ensure high translation efficiency and proper protein folding, using techniques like Western blot, ELISA, and fluorescence microscopy.
To support therapeutic development, we assess innate immune activation in primary immune cells or reporter assays, quantifying cytokine release and innate signaling markers.
In addition to conventional mRNA, we specialize in the production and validation of cutting-edge RNA modalities
Engineered for enhanced stability and prolonged expression, our circRNA synthesis service includes ligation, purification, and expression testing.
Our saRNA platform enables robust antigen expression at lower doses, ideal for vaccines and gene therapies. Services include replicon design, production, and functional assessment.
We produce high-fidelity gRNAs tailored for CRISPR/Cas applications, ensuring precise genome editing with minimized off-target effects.