We have extensive experience in the design and characterization of monoclonal antibodies (mAbs), bispecific antibodies (bsAbs), and more exotic biologics
Design and cloning of expression plasmids. Transfection of HEK293 or ExpCHO cells. Expression in 24-well, 96-well, shake flask, or library formats.
Measure Kon, Koff, or Kd to the antigen of your choice via BLI or SPR
Purity via SEC chromatography, Melting Temperature (Tm) via qPCR, aggregation via DLS, hydrophobicity via HIC chromatography
Screen large libraries for multi-hit binders via colony picking and NGS
Choose or design a reporter cell line to establish efficacy via immunofluorescence, western blot, or ELISA