mRNA Services

Core mRNA Services

From sequence optimization to functional validation

UTR Screening & Optimization

Evaluate and optimize 5' and 3' untranslated regions, CDS for enhanced stability and translation efficiency.

In vitro screening across multiple cell types for optimal expression profiles
Codon Optimization

Leverage proprietary algorithms to engineer mRNA with superior expression, greater stability, and low immunogenicity for therapeutic applications.

Context-specific analysis for mammalian expression systems
Plasmid Design & Construction

Custom plasmids with optimized regulatory elements and transcriptional controls.

High-yield mRNA production with your gene of interest

Production & Quality Control

In Vitro Transcription (IVT)

High-purity mRNA production using enzymatic IVT with customizable modifications:

Cap structures (Cap 0, Cap 1)
Modified nucleotides (pseudouridine, etc.)
Optimized poly(A) tails

RNA Purification & QC

Rigorous quality control for all mRNA batches:

HPLC for purity and modifications
Fragment Analyzer for integrity
UV spectrophotometry for yield
Gel electrophoresis confirmation

Validation & Advanced Formats

Functional Validation

Expression Testing: Western blot, ELISA, and fluorescence microscopy
Immunogenicity Profiling: Cytokine release and innate signaling assessment
Cell-Type Specific: Testing in relevant cell lines for your application

Advanced RNA Modalities

Circular RNA (circRNA): Enhanced stability and prolonged expression
Self-Amplifying RNA (saRNA): Robust expression at lower doses
Guide RNA (gRNA): High-fidelity CRISPR/Cas applications

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Why Choose ASE for mRNA Services?

Industry-leading expertise in therapeutic mRNA development with proven success in vaccine and gene therapy applications. Our integrated platform accelerates your path from concept to clinic.